Thursday, November 4, 2010

Vol. 16, No. 21

TRIGGER
The Twice-Monthly Electronic Newsletter on the Biology of Tissue Factor, Factor VII and TFPI
Vol. 16, No. 21  November 4, 2010
(The next issue will be on November 15, 2010)
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and BLOG:
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ANNOUNCEMENTS
ISTH Travel Awards for U.S. New Investigators - December 3 application deadline for U.S. New Investigators Travel Awards. These travel awards,to attend the ISTH Congress in Kyoto in July 2011, are anticipated to be $2,000, and will support Fellows and Junior Faculty at US laboratories. To be considered, applicants must submit an abstract and an NIH-style biosketch (2 pages). Applicants must also submit their abstracts in original or modified form to the Kyoto Congress abstract selection committee. ISTH 2011 Congress abstract instructions can be found at: http://www.isth2011.com/

Award selection will be announced by January 7, 2011. Receipt of the awards, which will be made on site at the Kyoto meeting, will also depend upon abstract acceptance by the Kyoto Congress. Candidates may go to the following web site by December 3 to submit their applications: http://www.kyototravelawards.org/

For questions about the awards, please email Dr. Kenneth Mann (Kenneth DOT Mann AT uvm DOT edu).
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PAPERS IN PRESS
AUTHORS: Choi SH, Collins JNR, Smith SA, Davis-Harrison RL, Rienstra CM, and Morrissey JH.
TITLE: Phosphoramidate end labeling of inorganic polyphosphates: Facile manipulation of polyphosphate for investigating and modulating its biological activities.
JOURNAL: Biochemistry, In Press.
ABSTRACT: Polyphosphates, linear polymers of inorganic phosphates linked by phosphoanhydride bonds, are widely present among organisms and play diverse roles in biology, including functioning as potent natural modulators of the human blood clotting system. However, studies of protein-polyphosphate interactions are hampered by a dearth of methods for derivatizing polyphosphate or immobilizing it onto solid supports. We now report that EDAC (1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide) efficiently promotes the covalent attachment of a variety of primary amine-containing labels and probes to the terminal phosphates of polyphosphates via stable phosphoramidate linkages. Using 31P NMR, we confirmed that EDAC-mediated reactions between primary amines and polyphosphate result in phosphoramidate linkages with the terminal phosphate groups. We show that polyphosphate can be biotinylated, labeled with fluorophores, and immobilized onto solid supports, that immobilized polyphosphate can be readily used to quantify protein binding affinities, that covalently derivatized or immobilized polyphosphate retains its ability to trigger blood clotting, and that derivatizing the ends of polyphosphate with spermidine protects it from exopolyphosphatase degradation. Our findings open up essentially the entire armamentarium of protein chemistry to modifying polyphosphate, which should greatly facilitate studies of its biological roles.
PREPRINT: http://pubs.acs.org/doi/abs/10.1021/bi1014437
SUBMITTED BY: Jim Morrissey (jhmorris AT illinois DOT edu)
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UPCOMING MEETINGS & EVENTS
Abstract Submission is now open for the Arteriosclerosis, Thrombosis and Vascular Biology 2011 Scientific Sessions, April 28-30, 2011 in Chicago Illinois, USA. Submission Deadline: Wednesday January 19, 2011. For more information:
http://www.americanheart.org/presenter.jhtml?identifier=3057022
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POSITIONS SOUGHT/AVAILABLE
Ph.D. and Technician Positions Available –
Are you a skilled protein biochemist looking for a new opportunity? Have you ever thought about moving to Vermont? If so, get your resume together and send it along! We're looking for technicians and a PhD level scientist experienced in protein biochemistry to add to our distinguished team at our facility in Essex Junction, Vermont USA. Experience working with blood coagulation proteins is preferred. Industry experience for both positions preferred. Check out the job postings here: http://www.haemtech.com/news.htm

Please, no phone calls. We're looking forward to hearing from you!
Robert Myers, Director, Business and Marketing Haematologic Technologies, Inc.
E-mail: rmyers AT haemtech DOT net
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RECENT PUBLICATIONS ON TISSUE FACTOR, FACTOR VII, AND TFPI
Papers are culled from NLM MedLine (PubMed). Titles in square brackets indicate that the paper was not published in English. References that lack volume or page numbers are typically electronic pre-publications (E-pubs) ahead of the print publication; if so, the final citation with volume and page numbers may or may not appear in a future issue of Trigger.
THE FOLLOWING LINK WILL TAKE YOU TO THE ENTIRE LIST OF THIS ISSUE'S PUBLICATIONS IN A PUBMED "COLLECTION":
http://www.ncbi.nlm.nih.gov/sites/myncbi/collections/public/1l51ojfq19nZSniwlaVFaMi/

Agmon-Levin N, Blank M, Zandman-Goddard G, Orbach H, Meroni PL, Tincani A, . . . Shoenfeld Y (2010) Vitamin D: an instrumental factor in the anti-phospholipid syndrome by inhibition of tissue factor expression. Ann Rheum Dis.
http://www.ncbi.nlm.nih.gov/pubmed/20980705

Alonso A, Tang W, Agarwal SK, Soliman EZ, Chamberlain AM, Folsom AR (2010) Hemostatic markers are associated with the risk and prognosis of atrial fibrillation: The ARIC study. Int J Cardiol.
http://www.ncbi.nlm.nih.gov/pubmed/20965585

Borensztajn K, Bresser P, van der Loos C, Bot I, van den Blink B, den Bakker MA, . . . Spek CA (2010) Protease-Activated Receptor-2 Induces Myofibroblast Differentiation and Tissue Factor Up-Regulation during Bleomycin-Induced Lung Injury. Potential Role in Pulmonary Fibrosis. Am J Pathol.
http://www.ncbi.nlm.nih.gov/pubmed/20971733

Breitenstein A, Stein S, Holy EW, Camici GG, Lohmann C, Akhmedov A, . . . Tanner FC (2010) Sirt1 inhibition promotes in vivo arterial thrombosis and tissue factor expression in stimulated cells. Cardiovasc Res.
http://www.ncbi.nlm.nih.gov/pubmed/20978007

Cho Y, Cao X, Shen D, Tuo J, Parver LM, Rickles FR, Chan CC (2010) Evidence for enhanced tissue factor expression in age-related macular degeneration. Lab Invest.
http://www.ncbi.nlm.nih.gov/pubmed/21042291

D'Angelo MR, Dutton RP (2010) Management of trauma-induced coagulopathy: trends and practices. AANA J 78:35-40.
http://www.ncbi.nlm.nih.gov/pubmed/20977127

Dreischaluck J, Schwoppe C, Spieker T, Kessler T, Tiemann K, Liersch R, . . . Berdel WE (2010) Vascular infarction by subcutaneous application of tissue factor targeted to tumor vessels with NGR-peptides: Activity and toxicity profile. Int J Oncol 37:1389-1397.
http://www.ncbi.nlm.nih.gov/pubmed/21042706

Girolami A, Bonamigo E, Vettore S (2010) The lack of ties between north-eastern Italy and African-Americans suggest a multi-founder effect for FVII Padua (Arg304Gln) disorder. Blood Coagul Fibrinolysis 21:775-776.
http://www.ncbi.nlm.nih.gov/pubmed/21042058

Girolami A, De Marinis GB, Bonamigo E, Allemand E (2010) Worldwide diffusion of FVII Arg304Gln coagulation defect (FVII Padua)((1)). Eur J Haematol.
http://www.ncbi.nlm.nih.gov/pubmed/20958793

Hillarp A, Baghaei F, Blixter IF, Gustafsson KM, Stigendal L, Sten-Linder M, . . . Lindahl TL (2010) Effects of the oral, direct factor Xa inhibitor rivaroxaban on commonly used coagulation assays. J Thromb Haemost.
http://www.ncbi.nlm.nih.gov/pubmed/20946166

Ittel A, Bretelle F, Gris JC, Chau C, Sebahoun G, Boubli L, Arnoux D (2010) Increased risk of gestational vascular complications in women with low free tissue factor pathway inhibitor plasma levels out of pregnancy. Thromb Haemost 105.
http://www.ncbi.nlm.nih.gov/pubmed/20978710

Kasthuri RS, Glover SL, Boles J, Mackman N (2010) Tissue factor and tissue factor pathway inhibitor as key regulators of global hemostasis: measurement of their levels in coagulation assays. Semin Thromb Hemost 36:764-771.
http://www.ncbi.nlm.nih.gov/pubmed/20978997

Klintman J, Astermark J, Berntorp E (2010) Combination of FVIII and by-passing agent potentiates in vitro thrombin production in haemophilia A inhibitor plasma. Br J Haematol 151:381-386.
http://www.ncbi.nlm.nih.gov/pubmed/20977448

Lehr EJ, Alford TJ, Wang SH (2010) Recombinant activated factor VII for postoperative hemorrhage following repair of acute type A aortic dissection. Heart Surg Forum 13:E275-279.
http://www.ncbi.nlm.nih.gov/pubmed/20961825

Matsuda S, Yamashita A, Sato Y, Kitajima S, Koike T, Sugita C, . . . Asada Y (2010) Human C-reactive protein enhances thrombus formation after neointimal balloon injury in transgenic rabbits. J Thromb Haemost.
http://www.ncbi.nlm.nih.gov/pubmed/21029360

Milligan C, Higginson I, Smith JE (2010) Emergency department staff knowledge of massive transfusion for trauma: the need for an evidence based protocol. Emerg Med J.
http://www.ncbi.nlm.nih.gov/pubmed/21030544

Schweintzger S, Schlagenhauf A, Rinner B, Bernhard H, Novak M, Leschnik B, Muntean W (2010) High microparticle concentration in cord plasma. Hamostaseologie 30:S122-125.
http://www.ncbi.nlm.nih.gov/pubmed/21042669

Sullivan BP, Weinreb PH, Violette SM, Luyendyk JP (2010) The Coagulation System Contributes to {alpha}V{beta}6 Integrin Expression and Liver Fibrosis Induced by Cholestasis. Am J Pathol.
http://www.ncbi.nlm.nih.gov/pubmed/21037076

Van Den Boogaard FE, Brands X, Schultz MJ, Levi M, Roelofs JJ, Van't Veer C, Van Der Poll T (2010) Recombinant Human Tissue Factor Pathway Inhibitor exerts anticoagulant, anti-inflammatory and antimicrobial effects in murine pneumococcal pneumonia. J Thromb Haemost.
http://www.ncbi.nlm.nih.gov/pubmed/21029363

Zhang M, Sun L (2010) The tissue factor pathway inhibitor 1 of Sciaenops ocellatus possesses antimicrobial activity and is involved in the immune response against bacterial infection. Dev Comp Immunol.
http://www.ncbi.nlm.nih.gov/pubmed/20970444

Zhang M, Xiao ZZ, Sun L (2010) Identification and analysis of the tissue factor pathway inhibitor 2 of Sciaenops ocellatus. Fish Shellfish Immunol.
http://www.ncbi.nlm.nih.gov/pubmed/20970505
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ABOUT TRIGGER
TRIGGER is edited by Jim Morrissey and Pierre Neuenschwander, with editorial assistance by Genie Morrissey. It includes Abstracts of Papers in Press, Meeting Announcements, Positions Sought/Available,or any other pertinent item that subscribers may want to share with their colleagues. To submit an item for publication in Trigger, e-mail us. To request or cancel a subscription,or for more information about TRIGGER or the biology of tissue factor and factor VII, visit our web site. You can also contact us by mail: J.H. Morrissey, University of Illinois College of Medicine, 417 Med Sci Bldg MC-714, 506 S. Mathews Ave., Urbana, IL 61801, USA.

DISCLAIMER: TRIGGER is provided "as is" for information purposes only and without warranty of any kind, express or implied. Users shall assume the entire risk as to the accuracy and usefulness of information in TRIGGER. Opinions expressed here are not necessarily those of the editors or their employers. You may share TRIGGER with your scientific colleagues but you are not permitted to use it or its contents for commercial purposes.
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