TRIGGER
The Twice-Monthly Electronic Newsletter on the Biology of Tissue Factor, Factor VII and TFPI
Vol. 16, No. 23 December 1, 2010
(The next issue will be on December 15, 2010)
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PAPERS IN PRESS
AUTHORS: Cho Y, Cao X, Shen DF, Tuo J, Parver LM, Rickles FR and Chan C-C.
TITLE: Evidence for enhanced tissue factor expression in age-related macular degeneration
JOURNAL: Laboratory Investigation
ABSTRACT: Tissue factor (TF) is the primary initiator of blood coagulation. In addition to hemostasis, TF can initiate intracellular signaling and promote inflammation and angiogenesis, the key processes underlying the pathogenesis of age-related macular degeneration (AMD). AMD, the leading cause of irreversible blindness among the elderly, involves many genetic and environmental risk factors, including oxidative stress and inflammation. In this study, TF expression was examined in human AMD tissue and in the eyes of a model of AMD, the Ccl2-/-/Cx3cr1-/-(DKO) mouse, as well as in the ARPE-19 cell line after lipopolysaccharide (LPS) and H2O2 stimulation. Total RNA was extracted from tissue samples and further analyzed by real-time RT-PCR. Immunohistochemistry was performed to evaluate TF protein expression. In the human retina, a 32-fold increase of TF mRNA expression was detected in AMD macular lesions compared with normal maculae. TF protein expression was also enhanced in human AMD maculae. Similarly, TF transcript and protein expression were moderately increased in retinal lesions, neuroretinal tissue, and cultured RPE cells of DKO mice compared with age-matched wild-type mice. TF expression level correlated with age in both wild-type and DKO mice. In order to better understand how AMD might lead to enhanced TF expression, 1, 5, and 10 mg/ml LPS as well as 100 and 200 mM H2O2 were used to stimulate ARPE-19 cells for 24 and 2 h, respectively. LPS treatment consistently increased TF transcript and protein expression. H2O2 alone or in combination with LPS also moderately enhanced TF expression. These results indicate that upregulated TF expression may be associated with AMD, and inflammatory and oxidative stress may contribute to TF expression in AMD eyes.
SUBMITTED BY: Frederick R. Rickles, M.D. (frickles AT mfa DOT gwu DOT edu)
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RECENT PUBLICATIONS ON TISSUE FACTOR, FACTOR VII, AND TFPI
Papers are culled from NLM MedLine (PubMed). Titles in square brackets indicate that the paper was not published in English. References that lack volume or page numbers are typically electronic pre-publications (E-pubs) ahead of the print publication; if so, the final citation with volume and page numbers may or may not appear in a future issue of Trigger.
THE FOLLOWING LINK WILL TAKE YOU TO THE ENTIRE LIST OF THIS ISSUE'S PUBLICATIONS IN A PUBMED "COLLECTION":
http://www.ncbi.nlm.nih.gov/sites/myncbi/collections/public/1Dqm8edJ8YqLi_hLPqyFAcv/
Agerso H, Brophy DF, Pelzer H, Martin EJ, Carr M, Hedner U, Ezban M (2010) Recombinant human factor VIIa (rFVIIa) cleared principally by antithrombin following IV administration in haemophilia patients. J Thromb Haemost.
http://www.ncbi.nlm.nih.gov/pubmed/21114621
Bajaj MS, Ogueli GI, Kumar Y, Vadivel K, Lawson G, Shanker S, . . . Bajaj SP (2010) Engineering Kunitz domain 1 (KD1) of human tissue factor pathway inhibitor-2 to selectively inhibit fibrinolysis: properties of KD1-L17R variant. J Biol Chem.
http://www.ncbi.nlm.nih.gov/pubmed/21115497
Celi A, Cianchetti S, Dell'omo G, Pedrinelli R (2010) Angiotensin II, tissue factor and the thrombotic paradox of hypertension. Expert Rev Cardiovasc Ther 8:1723-1729.
http://www.ncbi.nlm.nih.gov/pubmed/21108554
Dries DJ (2010) The contemporary role of blood products and components used in trauma resuscitation. Scand J Trauma Resusc Emerg Med 18:63.
http://www.ncbi.nlm.nih.gov/pubmed/21106098
Dusse LM, Carvalho MG, Cooper AJ, Lwaleed BA (2010) Plasma factor VII: A potential marker of pre-eclampsia. Thromb Res.
http://www.ncbi.nlm.nih.gov/pubmed/21094984
Girolami A, Berti de Marinis G, Bonamigo E, Sartori R, Vettore S (2010) Ox Brain versus Rabbit Brain Thromboplastin Assays Are the Best Tool for a Preliminary Diagnosis of the Arg304Gln Factor VII Defect (FVII Padua). Acta Haematol 124:229-234.
http://www.ncbi.nlm.nih.gov/pubmed/21099211
Jacob M, Chappell D, Stoeckelhuber M, Welsch U, Rehm M, Bruegger D, . . . Becker BF (2010) Perspectives in Microvascular Fluid Handling: Does the Distribution of Coagulation Factors in Human Myocardium Comply with Plasma Extravasation in Venular Coronary Segments? J Vasc Res 48:219-226.
http://www.ncbi.nlm.nih.gov/pubmed/21088428
Knudson MM, Cohen MJ, Reidy R, Jaeger S, Bacchetti P, Jin C, . . . Holcomb JB (2010) Trauma, Transfusions, and Use of Recombinant Factor VIIa: A Multicenter Case Registry Report of 380 Patients from the Western Trauma Association. J Am Coll Surg.
http://www.ncbi.nlm.nih.gov/pubmed/21115374
Lin Y, Stanworth S, Birchall J, Doree C, Hyde C (2010) Use of recombinant factor VIIa for the prevention and treatment of bleeding in patients without hemophilia: a systematic review and meta-analysis. CMAJ.
http://www.ncbi.nlm.nih.gov/pubmed/21078742
Liu X, Nie J, Guo SW (2010) Elevated immunoreactivity to tissue factor and its association with dysmenorrhea severity and the amount of menses in adenomyosis. Hum Reprod.
http://www.ncbi.nlm.nih.gov/pubmed/21115504
Owen BA, Xue A, Heit JA, Owen WG (2010) Procoagulant activity, but not number, of microparticles increases with age and in individuals after a single venous thromboembolism. Thromb Res.
http://www.ncbi.nlm.nih.gov/pubmed/21106230
Petersen LC, Karpf DM, Agerso H, Hermit MB, Pelzer H, Persson E, . . . Ezban M (2010) Intravascular inhibition of factor VIIa and the analogue NN1731 by antithrombin. Br J Haematol.
http://www.ncbi.nlm.nih.gov/pubmed/21083658
Scher C, Narine V, Chien D (2010) Recombinant factor VIIa in trauma patients without coagulation disorders. Anesthesiol Clin 28:681-690.
http://www.ncbi.nlm.nih.gov/pubmed/21074745
Shetty S, Bhave M, Ghosh K (2010) Acquired hemophilia A : diagnosis, aetiology, clinical spectrum and treatment options. Autoimmun Rev.
http://www.ncbi.nlm.nih.gov/pubmed/21115138
Temma T, Ogawa Y, Kuge Y, Ishino S, Takai N, Nishigori K, . . . Saji H (2010) Tissue factor detection for selectively discriminating unstable plaques in an atherosclerotic rabbit model. J Nucl Med 51:1979-1986.
http://www.ncbi.nlm.nih.gov/pubmed/21078793
Zakai NA, Lange L, Longstreth WT, O'Meara ES, Kelley JL, Fornage M, . . . Reiner AP (2010) Association of coagulation and inflammation related genes and factor VIIc levels with stroke: the cardiovascular health study. J Thromb Haemost.
http://www.ncbi.nlm.nih.gov/pubmed/21114618
Zhou H, Yan Y, Xu G, Zhou B, Wen H, Guo D, . . . Wang H (2010) Toll-like receptor (TLR)-4 mediates anti-beta(2) GPI/beta(2) GPI-induced tissue factor expression in THP-1 cells. Clin Exp Immunol.
http://www.ncbi.nlm.nih.gov/pubmed/21091668
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ABOUT TRIGGER
TRIGGER is edited by Jim Morrissey and Pierre Neuenschwander, with editorial assistance by Genie Morrissey. It includes Abstracts of Papers in Press, Meeting Announcements, Positions Sought/Available,or any other pertinent item that subscribers may want to share with their colleagues. To submit an item for publication in Trigger, e-mail us. To request or cancel a subscription,or for more information about TRIGGER or the biology of tissue factor and factor VII, visit our web site. You can also contact us by mail: J.H. Morrissey, University of Illinois College of Medicine, 417 Med Sci Bldg MC-714, 506 S. Mathews Ave., Urbana, IL 61801, USA.
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